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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -PBEF (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor, 1110035O14Rik, DKFZP666B131, EC 2.4.2.12, MGC117256, Nicotinamide Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1, Visfatin)

Anti -PBEF (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor,
1110035O14Rik, DKFZP666B131, EC 2.4.2.12, MGC117256, Nicotinamide
Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1,
Visfatin)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
Visfatin is pre-B cell colony-enhancing factor 1(PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.
Catalog #P3113-97N
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA (Direct and Indirect): 1:2000-1:5000
Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Incubation time: 1 hour at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified immunoglobulin
ImmunogenSynthetic peptide corresponding to aa40-56, R40EKKTENSKLRKVKYEE56 of human Visfatin.
SpecificityRecognizes human Visfatin. Species Crossreactivity: Weakly crossreacts with mouse Visfatin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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