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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -PBEF (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor, 1110035O14Rik, DKFZP666B131, EC 2.4.2.12, MGC117256, Nicotinamide Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1, Visfatin) (Biotin)

Anti -PBEF (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor,
1110035O14Rik, DKFZP666B131, EC 2.4.2.12, MGC117256, Nicotinamide
Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1,
Visfatin) (Biotin)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase) is a 52kD member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97% and 96% aa identity with mouse, porcine and canine PBEF, respectively.
Catalog #P3113-97T
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml detection limit for rhPBEF is ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in Tris, pH 7.3, 1% BSA. Reconstitute with 1ml sterile 40-50% glycerol, Tris, 0.1mg/ml BSA. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived rhPBEF. (aa 27 - 491)
SpecificityRecognizes human PBEF.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesNicotinamide Phosphoribosyltransferase


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