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You are here:Home » Antibodies » Antibodies-Nuclear Proteins » Anti -PCNA (Proliferating Cell Nuclear Antigen)

Anti -PCNA (Proliferating Cell Nuclear Antigen)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfill several separate roles in the cell nucleus associated with changes in its antigenic structure.
Catalog #P3115-02A
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (FFPE): 1:40 for biotin based detection. Pretreatment is not required. For optimal staining, incubate the primary antibody for 20-60 minutes at RT.
Optimal dilutions to be determined by the researcher.
Positive ComtrolsHuman colon or tonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
Clone No6D645
ConcentrationNot Determined
FormSupplied as a liquid in 0.01M PBS, 1% BSA, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant rat PCNA (proliferating cell nuclear antigen).
SpecificityRecognizes PCNA from all vertebrate species, insects and S. pombe. Intense nuclear staining of proliferating cells in normal and tumor tissues.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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