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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -PCNA (Proliferating Cell Nuclear Antigen)

Anti -PCNA (Proliferating Cell Nuclear Antigen)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.
Catalog #P3115-11B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000. Detects a band at 30kD.
Immunohistochemistry (Paraffin Embedded): 10ug/ml. Heat-mediated citrate buffer antigen retrieval.
Optimal dilutions to be determined by the researcher
Postive ControlDT40 B lymphocyte whole cell lysate (chicken)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50% ) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3C169
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein A chromatography.
ImmunogenProliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein.
SpecificityRecognizes rat PCNA p36 protein expressed at high levels in proliferating cells. Species crossreactivity: human, mouse, yeast
(S. pombe and S. cerevisiae), chicken and Drosophila melanogaster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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