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You are here:Home » Antibodies » Antibodies-Enzymes, Isomerase » Anti -PDI (Protein Disulfide Isomerase)

Anti -PDI (Protein Disulfide Isomerase)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC
The mammalian protein disulfide-isomerase (PDI) family encompasses several highly divergent proteins which are involved in the processing and maturation of secretory proteins in the ER by catalyzing the rearrangement of disulfide bonds (2,7). PDI, which is an abundant protein of the ER (> 400uM), has a carboxy-terminal retention signal sequence, KDEL, similar to that of BiP and Grp94 (1). The PDI proteins are characterized by the presence of one or more domains of ~95-110 amino acids related to the cytoplasmic protein thioredoxin (2). All but the PDI-D subfamily are composed entirely of repeats of such domains, with at least one domain containing and one domain lacking a redox-active-Cys-Xaa-Xaa-Cys-tetrapeptide (2). In addition to their roles as redox catalysts and isomerases, PDI proteins have other functions such as peptide binding, cell adhesion and perhaps chaperone activities (2). Platelet surface thiols and disulfides play an important role in platelet responses (3). Catalytically active PDI is found on platelet surfaces where it has been demonstrated to mediate platelet aggregation and secretion possible by reducing disulfide bonds thus leading to exposure of fibrinogen receptors in platelets (3).
Catalog #P3122-05
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionsWestern Blot (Colorimetric): 1:1000
Optimal dilutions to be determined by researcher.
Positive Controls:
Purified Bovine PDI Protein
HeLa Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified bovine liver Protein Disulfide Isomerase (PDI).
SpecificityRecognizes bovine PDI protein. Detects an ~58kD protein, corresponding to the apparent molecular mass of PDI on SDS-PAGE immunoblots. Species Crossreactivity: Human, mouse, rat, monkey, hamster, guinea pig, bovine, sheep, porcine, canine and Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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