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You are here:Home » Antibodies » Abs to Enzymes, Isomerase » Anti -P4HB (Protein Disulfide-isomerase, PDI, Prolyl 4-hydroxylase Subunit beta, Cellular Thyroid Hormone-binding Protein, p55, PO4DB, PDIA1, PDI, ERBA2L)

Anti -P4HB (Protein Disulfide-isomerase, PDI, Prolyl 4-hydroxylase Subunit beta, Cellular
Thyroid Hormone-binding Protein, p55, PO4DB, PDIA1, PDI, ERBA2L)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
P4HB is a 60kD member of the protein disulfide isomerase family. As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4-hydrolyase. This catalyses the hydroxylation of proline in collagen. On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells. Mature human P4HB is 491aa in length. It contains two TRX domains aa25-134 and 368-475 plus an ER retention sequence aa505-508. There is one potential isoform that shows an 11aa substitution for the first 162aa. Over aa18-505, human P4HB shares 94% aa identity with mouse P4HB.
Catalog #P3122-05G
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2A
Clone No11n150
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa18-505 from human Protein Disulfide Isomerase/P4HB expressed in CHO cells (P07237).
SpecificityRecognizes human P4HB. Species sequence homology: mouse (94%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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