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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH IF

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins (3).
Catalog #	P3122-11
Applications	Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:1000
	Immunohistochemistry (Paraffin): 1:100. Antigen retrieval in citrate buffer required.
	Immunofluorescence (IF-IC): 1:50; Requires methanol permeabilization.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity	Purified by Protein A and peptide affinity chromatography.
Immunogen	Synthetic peptide corresponding to residues surrounding Pro329 of human PDI.
Specificity	Recognizes endogenous levels of total human PDI protein at ~57kD. Species Crossreactivity: monkey, mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.