| Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulfide-bond formation and isomerization, as well as the reduction of disulfide bonds in proteins. PDI, which catalyses disulfide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulfide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulfide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94kD glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
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| Catalog # | P3122-15 |
| Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000; Detects a protein at 59kD representing PDI from rat liver extract or a slightly higher protein at 61kD representing PDI from human liver extract. |
| Immunoprecipitation: Shown to inhibit the activity of PDI in vitro and has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells. |
| Immunohistochemistry (Frozen, FFPE): 1:100 Staining of rat intestine yields cytoplasmic staining. |
| Immunofluorescence: 1:100 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 4i387 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Full length purified native rat PDI |
| Specificity | Recognizes rat PDI. Species Crossreactivity: human, mouse, porcine, hamster. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
