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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -PDK-1 (Pyruvate Dehydrogenase Kinase-1, PDHK1)

Anti -PDK-1 (Pyruvate Dehydrogenase Kinase-1, PDHK1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Pyruvate dehydrogenase kinase 1 (PDK1), a 48kD member of
the mitochondrial matrix protein kinase family, is related to the
histidine protein kinases found in prokaryotes. A mitochondrial multiple subunit enzyme, pyruvate dehydrogenase (PDH) catalyses the oxidative decarboxylation of pyruvic acid to generate acetyl-CoA, NADH, and CO2. This reaction is central to the homeostasis of carbohydrate fuels in mammals. Along with three other isoforms, PDK1 is responsible for phosphorylation and concomitant inactivation of PDH, the E1 component of the multi-enzyme pyruvate dehydrogenase complex. Phosphorylation of PDH by PDK1 occurs at three serine residues on the E1 alpha subunits: Ser264, Ser271, and Ser203. In vitro studies indicate that PDK1 is the only isoenzyme able to phosphorylate Ser203 of PDH, while PDK2 and PDK3 exhibit the strongest activity toward Ser264 and Ser271, respectively. Northern blot analysis reveals that PDK1 is predominantly expressed in heart tissue.
Catalog #P3123-10
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot (ECL): 1:1000
Immunohistochemistry (Paraffin): 10ug/ml
Optimal dilutions to be determined by researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to a sequence from human PDK-1, conjugated to KLH.
SpecificityRecognizes human PDK-1 at ~48kD.
Species CrossreactivityMouse, rat, canine, monkey, porcine and rabbit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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