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Anti -PEDF (Pigment Epithelial Derived Factor)
Anti -PEDF (Pigment Epithelial Derived Factor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH IC |
|
| Pigment epithelium-derived factor (PEDF) was first identified as a 50kD secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells. More recently, PEDF was shown to have neurotrophic activity in the retina in vivo. PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells. The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. PEDF has been shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo. | | | Catalog # | P3140-01 | | Applications | Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. | | Recommended Dilutions | Western Blot: 1:1000 detects pigment epithelium on 10ug of human placenta lysate. | | Immunohistochemistry: Frozen sections | | Optimal dilutions to be determined by the researcher. | | Positive Control | Eye tissue | | Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 2Q1840 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant corresponding to aa196-347 of human PEDF fusion protein tagged with GST on the N-terminal was expressed in E. coli. Species Sequence Homology: rat. | | Specificity | Recognizes human PEDF at ~46kD. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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