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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -PEDF (Pigment Epithelial Derived Factor)

Anti -PEDF (Pigment Epithelial Derived Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IC
Pigment epithelium-derived factor (PEDF) was first identified as a 50kD secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells. More recently, PEDF was shown to have neurotrophic activity in the retina in vivo. PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells. The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. PEDF has been shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo.
Catalog #P3140-01
ApplicationsSuitable for use in Western Blot, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1000 detects pigment epithelium on 10ug of human placenta lysate.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Positive ControlEye tissue
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q1840
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant corresponding to aa196-347 of human PEDF fusion protein tagged with GST on the N-terminal was expressed in E. coli. Species Sequence Homology: rat.
SpecificityRecognizes human PEDF at ~46kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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