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Anti -PEDF (Pigment Epithelium Derived Factor, Alpha-2-antiplasmin, EPC-1, Proliferation-inducing Protein 35, PIG35, Serpin Peptidase Inhibitor Clade F Member 1, SERPINF1, SERPIN F1)
Anti -PEDF (Pigment Epithelium Derived Factor, Alpha-2-antiplasmin, EPC-1,
Proliferation-inducing Protein 35, PIG35, Serpin Peptidase Inhibitor Clade F Member 1,
SERPINF1, SERPIN F1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH |
|
| Catalog # | P3140-02D | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-2ug/ml. 40ug of Y79 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti PEDF (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Positive Control | RIPA lysates from Y79 cells. | | Cellular Localization | Secreted | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | His tagged fusion protein corresponding to full length human PEDF (Pigment Epithelial Derived Factor) expressed in baby hamster kidney cells. | | Specificity | Recognizes PEDF at 50kD. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms. Species Crossreactivity: Crossreacts with Human and Mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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