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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -PEDF (Pigment Epithelium Derived Factor, Alpha-2-antiplasmin, EPC-1, Proliferation-inducing Protein 35, PIG35, Serpin Peptidase Inhibitor Clade F Member 1, SERPINF1, SERPIN F1)

Anti -PEDF (Pigment Epithelium Derived Factor, Alpha-2-antiplasmin, EPC-1,
Proliferation-inducing Protein 35, PIG35, Serpin Peptidase Inhibitor Clade F Member 1,
SERPINF1, SERPIN F1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
PEDF R (Pigment epithelium derived factor receptor; also ATGL, PNPLA2 and desnutrin) is a 55kD member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Mouse PEDF R is described as being a 486aa type II transmembrane
(TM) protein. Its TM segment is stated to span aa9-29. Alternatively, it may have a hydrophobic lipid-association domain between aa 267-295. It contains a palatin domain (aa10-179) with a conserved GxSxG enzymatic motif. There are multiple splice forms. Two show alternate start sites at Met57 and Met232, while two others show a deletion of aa163-196 and 253-308,
respectively. Over aa 62-253, mouse PEDF R shares 98% and 93% aa identity with rat and human PEDF R, respectively.
Catalog #P3140-02D
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml. 40ug of Y79 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti PEDF (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Positive Control RIPA lysates from Y79 cells.
Cellular Localization Secreted
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenHis tagged fusion protein corresponding to full length human PEDF (Pigment Epithelial Derived Factor) expressed in baby hamster kidney cells.
SpecificityRecognizes human PEDF at 50kD. Additional bands were detected at 66 and 76kD, which were competely taken away by incubation with the immunogen. These bands may be post translationally modified forms. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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