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You are here:Home » Molecular Biology » MB-Protease Inhibitors » PEDF, Recombinant, Human (Pigment Epithelium Derived Factor, Alpha-2-antiplasmin, EPC-1, Proliferation-inducing Protein 35, PIG35, Serpin Peptidase Inhibitor Clade F Member 1, SERPINF1, SERPIN F1)

PEDF, Recombinant, Human (Pigment Epithelium Derived Factor, Alpha-2-antiplasmin, EPC-1,
Proliferation-inducing Protein 35, PIG35, Serpin Peptidase Inhibitor Clade F Member 1,
SERPINF1, SERPIN F1)

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Specifications

PEDF, also known as SERPINF1, is an extracellular neurotrophic agent, first identified as a secreted product in conditioned medium from cultured fetal human retinal pigment epithelial (RPE) cells. It was reported that PEDF isolated from medium conditioned by human fetal RPE primary cultures promotes neurite outgrowth in cultured human retinoblastoma Y-79 cells. PEDF is also a member of the serpin family, a group of serine protease inhibitors. As PEDF does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Catalog #P3140-02E
SourceRecombinant PEDF protein containing a natural variant M72T was expressed in E.coli and purified by using conventional chromatography techniques.
Molecular Weight46.7kD(420aa).
AA SequenceMGSSHHHHHH SSGLVPRGSH MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP
Storage and StabilityFor long-term storage, aliquot and store at -20°C/-70°C. Aliquots are stable for at least 6 months at -20°C/-70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity~85% by SDS-PAGE.
ConcentrationAs reported
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH8, 20% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEPC-1, PIG35, SERPINF1


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