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You are here:Home » Antibodies » Abs to Neurotrophins » Anti -PEDF R (Pigment Epithelial Derived Factor Receptor)

Anti -PEDF R (Pigment Epithelial Derived Factor Receptor)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
PEDF R (Pigment epithelium derived factor receptor; also ATGL, PNPLA2 and desnutrin)is a 55kD member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle, and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Mouse PEDF R is described as being a 486aa type II transmembrane (TM) protein. Its TM segment is stated to span (aa9-29). Alternatively, it may have a hydrophobic lipid-association domain between (aa267-295). It contains a palatin domain (aa10-179) with a conserved GxSxG enzymatic motif. There are multiple splice forms. Two show alternate start sites at Met57 and Met232, while two others show a deletion of (aa163-196) and (aa253-308), respectively. Over (aa162-253), mouse PEDF R shares 98% and 93% aa identity with rat and human PEDF R, respectively.
Catalog #P3140-04
ApplicationsSuitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaMyeloma cells with B cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No11D53
HostRat
SourceMouse
Concentration~0.5mg/ml (after reconstitition)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa162-253 from mouse PEDF R expressed in E. coli (Q8BJ56).
SpecificityRecognizes mouse PEDF R.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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