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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Pepsin

Anti -Pepsin

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Specifications

Clone Host Grade Applications
Polyclonal Goat Serum E B IP Gel
epsin, an acidic protease, is the principal proteolytic enzyme of vertebrate gastric juice. Its inactive precursor, pepsinogen, is produced in stomach mucosa. Pepsin has a broad range of substrates and demonstrates an esterase activity. There are several minor pepsins designated B, C, and D; the major component is A to which the following data applies. They are chromatographically separable.
Catalog #P3270-05
Conversion of pepsinogen to pepsin, which involves releasing a peptide, may be an autocatalytic process involving self-cleavage by the zymogen. Peptides released on activation have pepsin inhibitory activity.
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA, and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionELISA: 1:3000-1:11,000 using 1ug in a standard sandwich ELISA using peroxidase conjugated affinity pure goat IgG [H&L] (rabbit) and ABTS as a substrate for 30 min at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourcePorcine
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, 0.01% sodium azide, 40% glycerol.
PurityPurified by delipidation and de brination.
ImmunogenPepsin (Porcine stomach)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified pepsin (porcine stomach).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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