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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -PGA5 (Pepsin A-5, Pepsinogen-5)

Anti -PGA5 (Pepsin A-5, Pepsinogen-5)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens and then converted to active pepsins by the acidic pH of gastric juices. PGA 3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 2-4aa. Human PGA5 cDNA encodes 388aa including a 15aa signal sequence, a 47aa activation peptide, and a 326aa active enzyme. Human pepsinogen A isozymogens share ~56% aa sequence identity with mouse and rat pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin. Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic aa's.
Catalog #P3270-05E
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.2ug/ml
Immunohistochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No12K160
HostMouse
SourceHuman
Concentration~0.5mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa16-388 from human PGA5 expressed in NSO cell line.
SpecificityRecognizes human PGA5. No crossreactivity with recombinant human Pepsinogen C, rhCathepsin D, or rhCathepsin E.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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