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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Pepsin

Anti -Pepsin

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified E B IH
Pepsin, an acidic protease, is the principal proteolytic enzyme of vertebrate gastric juice. Its inactive precursor, pepsinogen, is produced in stomach mucosa. Historically it is of interest as one of the first enzymes to be crystallized. There are several pepsins designated A, B, C and D. Pepsin A, the major component, has a MW of 35kD. Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine and tyrosine. It will not cleave at bonds containing valine, alanine or glycine.
Catalog #P3270-06
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:50,000- 1:200,000
Western Blot: 1:5000-1:20,000
Immunohistochemistry: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourcePorcine
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPuri ed by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenPepsin (Porcine Stomach).
SpecificityRecognizes porcine Pepsin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purufied and partially purified pepsin (porcine stomach).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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