Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Pepsin (HRP)

Anti -Pepsin (HRP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified E B IH
epsin, an acidic protease, is the principal proteolytic enzyme of vertebrate gastric juice. Its inactive precursor, pepsinogen, is produced in stomach mucosa. Pepsin has a broad range of substrates and demonstrates an esterase activity. There are several minor pepsins designated B, C, and D; the major component is A to which the following data applies. They are chromatographically separable.
Catalog #P3270-15
Conversion of pepsinogen to pepsin, which involves releasing a peptide, may be an autocatalytic process involving self-cleavage by the zymogen. Peptides released on activation have pepsin inhibitory activity.
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourcePorcine
Concentration~5mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
PurityPuri ed by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenPepsin (Porcine Stomach).
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxide, anti-goat serum and purified and partially purified Pepsin (Porcine Stomach).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links