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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IP IH IC FC

Perforin, originally cloned from mouse cytotoxic T-lymphocyte (CTL), is a 555-amino acid protein with a 21-amino acid signal peptide and is a potent cytolytic pore-forming protein. It is a specific marker of functionally active cytotoxic T-lymphocytes (CTLs) and natural killer (NK) cells. Fas ligand and perforin are the two key effector mechanisms in T cell-mediated cytotoxicity. These molecules mediate cytolysis of target cells by membrane damage and apoptosis, an important mechanism in the immune defense against tumors and virus infection. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation.
Catalog #	P3340-05
Applications
	Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Immunoprecipitation: 2-8ug/ml
	Flow Cytometry: 2-8ug/ml
	Immunohistochemistry (frozen and paraffin sections): 2-8ug/ml
	Immunocytochemistry.: 2-8ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	1.VB.3
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 1mg/ml BSA, 1.5mM thimerosal, 50% glycerol.
Purity	Purified.
Immunogen	Purified granules from human YT lymphoma cells.
Specificity	Recognizes human perforin at ~70kD. Species Crossreactivity: primate.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.