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P3340-98B1 Sheep Anti-Perilipin (PERI, PLIN, Lipid Droplet-associated Protein)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Human
Swiss Prot
O60240
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Perilipin is a 62kD constitutively phosphorylated protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules. Found primarily in mature adipocytes, perilipin-1 interacts with lipid-coat proteins at the edge of the fat droplet and blocks lipase activity. Upon phosphorylation, perilipin-1 becomes a 65-67kD molecule that likely promotes the dispersion of docking molecules and provides a scaffold for lipase at the lipid droplet surface. Human perilipin-1 is 522aa in length. It contains a lipid droplet targeting region aa233-364 that contains a polyGlu segment aa307-316. Phosphorylation at the N-terminus is necessary for lipase interaction with lipid. One 50-54kD human splice variant (perilipin-B) is reported that shows an 85aa substitution for aa404-522. This apparently interacts with cell membrane triglycerides. Over aa8-145, human perilipin-1/A shares 97aa identity with mouse perilipin-1.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa8-145 from human Perilipin expressed in E. coli (O60240).
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Perilipin.
Conjugates
Pricing
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