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Anti -Perilipin (PERI, PLIN, Lipid Droplet-associated Protein)
Anti -Perilipin (PERI, PLIN, Lipid Droplet-associated Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| Perilipin is a 62kD constitutively phosphorylated protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules. Found primarily in mature adipocytes, perilipin-1 interacts with lipid-coat proteins at the edge of the fat droplet and blocks lipase activity. Upon phosphorylation, perilipin-1 becomes a 65-67kD molecule that likely promotes the dispersion of docking molecules and provides a scaffold for lipase at the lipid droplet surface. Human perilipin-1 is 522aa in length. It contains a lipid droplet targeting region aa233-364 that contains a polyGlu segment aa307-316. Phosphorylation at the N-terminus is necessary for lipase interaction with lipid. One 50-54kD human splice variant (perilipin-B) is reported that shows an 85aa substitution for aa404-522. This apparently interacts with cell membrane triglycerides. Over aa8-145, human perilipin-1/A shares 97aa identity with mouse perilipin-1. | | | Catalog # | P3340-98B1 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa8-145 from human Perilipin expressed in E. coli (O60240). | | Specificity | Recognizes human Perilipin. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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