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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Perilipin

Anti -Perilipin

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum B IH
Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, perilipin A and B arise by alternative RNA splicing from a single gene, generating polypepides of 57 and 46kD, respectively. The N-terminus, however, remains unchanged. The antiserum recognizes perilipins (A and B)
located at the surface of intracellular storage lipid droplets present, e.g. in the adrenal gland, adipocytes of white and brown adipose tissue, and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells.
Catalog #P3340-99
ApplicationsSuitable for use in Western Blot and Immunohistochemistry Other applications have not been tested.
Recommended DilutionWestern Blot (ECL): 1:2000
Immunohistochemistry: 1:100-1:200 for 1 hour at RT on paraffin sections after microwave treatment or on frozen sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGuinea pig
ConcentrationNot Determined
FormSupplied as a liquid, 0.09% sodium azide.
PuritySerum
ImmunogenDuplicated N-terminus of perilipin
SpecificityRecognizes perilipins (A and B) at ~57 and ~46kD, respectively. Does not cross-react with ADRP (adipophilin) or TIP47 proteins. Species Crossreactivity: human, rat and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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