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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Serum B IH

Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, perilipin A and B arise by alternative RNA splicing from a single gene, generating polypepides of 57 and 46kD, respectively. The N-terminus, however, remains unchanged. The antiserum recognizes perilipins (A and B)
located at the surface of intracellular storage lipid droplets present, e.g. in the adrenal gland, adipocytes of white and brown adipose tissue, and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells.
Catalog #	P3340-99
Applications	Suitable for use in Western Blot and Immunohistochemistry Other applications have not been tested.
Recommended Dilution	Western Blot (ECL): 1:2000
	Immunohistochemistry: 1:100-1:200 for 1 hour at RT on paraffin sections after microwave treatment or on frozen sections.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Concentration	Not Determined
Form	Supplied as a liquid, 0.09% sodium azide.
Purity	Serum
Immunogen	Duplicated N-terminus of perilipin
Specificity	Recognizes perilipins (A and B) at ~57 and ~46kD, respectively. Does not cross-react with ADRP (adipophilin) or TIP47 proteins. Species Crossreactivity: human, rat and mouse
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.