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You are here:Home » Antibodies » Abs to Circadian Proteins » Anti -Period Clock Protein 1 (Per1, RIGUI)

Anti -Period Clock Protein 1 (Per1, RIGUI)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Recently human and mouse genes encoding a basichelix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported. The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively. Homologues of mPer1 designated Per 2 (1257aa) and Per3 (1113aa) have also been cloned. Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes. It has been suggested that mPer regulates neuronal activity in the SCN. Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice. Clock has been mapped to chromosome 5. Mouse Clock encodes a transcription factor, a single polypeptide chain of 855aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846aa). Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney). Although, Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery.
Catalog #P3342-01C
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000.
ELISA 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide P3342-12A
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid, neat serum, 0.05% sodium azide, 40% glycerol.
PuritySerum
ImmunogenA 16-aa peptide internal sequence mapping downstream of the SG repeat of mouse Per 1 (KLH conjugated).
SpecificityRecognizes Period Clock Protein 1. No significant homology is seen with Per2, Per 3, dPER or other known proteins. Species homology: human Per1 (93%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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