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You are here:Home » Antibodies » Abs to Circadian Proteins » Anti -Period Clock Protein 2 (PER2)

Anti -Period Clock Protein 2 (PER2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Catalog #P3342-20B
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
PuritySerum
ImmunogenA 23-aa peptide sequence within C-terminus of mouse Per2 (1) was synthesized coupled to KLH
SpecificityThe mouse peptide sequence is 77% conserved in rat Per2. No significant homology is seen with human Per2 or Per1 or Per3. Antibody crossreactivity with Per2 from other species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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