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You are here:Home » Antibodies » Abs to Peripherin » Anti -Peripherin (PRPH, Neurofilament 4, NEF4)

Anti -Peripherin (PRPH, Neurofilament 4, NEF4)

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Specifications

Clone Host Grade Applications
Rabbit Serum B IH
Peripherin is a 56-58kD type III intermediate filament protein, expressed in motor, sensory and sympathetic system neurons as well as neuroendocrine skin carcinomas and certain melanocytic tumors. It is often used an indicator of enteric ganglion cell density in normal and diseased (e.g. Hirschsprung's disease) states. Abnormal inclusions containing peripherin have also been reported in ALS. During normal, late neurogenesis peripherin appears after the expression of nestin, vimentin, alpha-internexin and neurofilaments and is involved in the differentiation of neurons, namely of the peripheral nervous system, and also probably in axonal regeneration. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.
Catalog #P3345-01
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and Electron Microscopy. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Frozen, paraffin): 1:100-1:200 Fix frozen sections in acetone at -20°C. Works on tissue fixed for one hour or less in fresh 4% paraformaldehyde.
Western Blot: 1:1000 detected Peripherin on 10ug PC12 lysates. PC12 lysate was resolved by electrophoresis, transferred to PVDF membrance and probed with P3345-01. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
ControlRat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
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