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You are here:Home » Antibodies » Antibodies-Peripherin » Anti -Peripherin (PRPH, Neurofilament 4, NEF4)

Anti -Peripherin (PRPH, Neurofilament 4, NEF4)


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Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IH IC
Peripherin is a Class III intermediate filament subunit found in both the peripheral and central nervous systems, though it is concentrated, as its name suggests, in the neurons of peripheral ganglia and their processes. Antibodies to peripherin can be used in identifying, classifying, and studying neurons in the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig’s disease and some neuronally derived tumors. Peripherin is also a major component of the cytoskeleton of PC12 cells, a widely used model system for studies of neuronal differentiation. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.
Catalog #P3345-03
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:100
Immunohistochemistry: Frozen, unfixed sections
Immunohistochemistry: Formalin fixed paraffin embedded sections. Keep fixation time short, i.e., less than 10 minutes in formalin, followed by PBS wash.
Immunocytochemistry: 1:25-1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3C170
ConcentrationNot determined
FormSupplied as a liquid containing sodium azide.
PurityTissue culture supernatant
ImmunogenRecombinant peripherin
SpecificityRecognizes human peripherin. Species Crossreactivity: Reacts with all mammalian species tested to date.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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