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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -PGC-1 alpha, phosphorylated (S571) (Peroxisome Proliferator Activated Receptor gamma Coactivator 1 alpha, PPAR gamma Coactivator 1 alpha, PPARGC-1 alpha, PPARGC1A, PGC1 alpha, PGC1A, PGC1, PGC-1, Ligand Effect Modulator 6, LEM6)

Anti -PGC-1 alpha, phosphorylated (S571) (Peroxisome Proliferator Activated Receptor gamma
Coactivator 1 alpha, PPAR gamma Coactivator 1 alpha, PPARGC-1 alpha, PPARGC1A, PGC1 alpha,
PGC1A, PGC1, PGC-1, Ligand Effect Modulator 6, LEM6)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
PGC-1a is a 97-120kD member of the PGC-1 family of proteins. It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes. PGC-1a participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPARg, RAR and TR. Human PCG-1a is 798aa in length. It contains an LxxLL nuclear receptor binding motif aa144-148, one PPAR-g interaction domain aa293-339, two NLSs and an RNA binding/processing region aa566-710. PGC-1a activity is regulated by phosphorylation. AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity. Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC-1a activity. This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG-1a acetylation that promotes PGC-1a dissociation from target gene promoters.
Catalog #P3363-03C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml using HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line treated with recomninant human IGF-1
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose, 0.1% sodium azide. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenPhosphopeptide containing the human PGC1a S571 site.
SpecificityRecognizes human PGC1 alpha, when phosphorylated at S571.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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