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P3368-01N Goat Anti-PHAPI2 (Acidic Leucine-rich Nuclear Phosphoprotein 32 Family Member B, Acidic Protein Rich in Leucines, Putative HLA-DR-associated Protein I-2, ANP32B, Silver-stainable Protein SSP29, APRIL)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Bo Ca Hu Mo Po Rt
Accession #
NP_006392.1
Gene ID
10541, 67628, 170724
Shipping Temp
Blue Ice
Storage Temp
-20°C

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity. Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1000 Western Blot: 0.3-1ug/ml, human lymph node lysates and in lysates of cell line A431 observed on ~26kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-KRKRETDDEGEDD, from human SSP29, at C-terminal (NP_006392.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SSP29. Species sequence homology: Bovine, canine, mouse, porcine and rat.
References
1. Munemasa Y, Suzuki T, Aizawa K, Miyamoto S, Imai Y, Matsumura T, Horikoshi M, Nagai R.|Promoter region-specific histone incorporation by the novel histone chaperone ANP32B and DNA-binding factor KLF5. Mol Cell Biol. 2008 Feb;28(3):1171-81.
USBio References
No references available
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