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You are here:Home » Antibodies » Abs to Hypoxia » Anti -PHD1 (Prolyl Hydroxylase Domain-containing Protein 1, PHD-1, DKFZp434E026, EGL Nine (C.elegans) Homolog 2, Egl Nine Homolog 2, EGLN2, Estrogen-induced Tag 6, EIT6, EIT-6, Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF Prolyl Hydroxylase 1, HIF-PH1, HI

Anti -PHD1 (Prolyl Hydroxylase Domain-containing Protein 1, PHD-1, DKFZp434E026, EGL Nine
(C.elegans) Homolog 2, Egl Nine Homolog 2, EGLN2, Estrogen-induced Tag 6, EIT6, EIT-6,
Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF Prolyl Hydroxylase 1, HIF-PH1, HI

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IP
EGLN2 (EGL/EGg LayingNine #2 also known as PHD1 and HPH3) is a 43kD member of the EglN family of proteins. It is ubiquitously expressed and found principally in the nucleus. EGLN2 hydroxylates proline on HIF1 a. HIF1 is an a/b heterodimeric transcriptional activa that up
regulates genes involved in mitigating the effects of hypoxia. Normally, and in the presence of abundant oxygen, the HIF 1 a chain is hydroxylated by PHD family members, which results in its
ubiquitylation and degradation. Under low oxygen tension, EGLN2 activity is decreased, the HIF1 a subunit is retained, and HIF1 activates genes. Human EGLN2 is 407aa in length. It contains one iron 2oxoglutarate (Fe2OG) dioxygenase domain (aa278-376) plus an ironbinding (His297 His358), and a 2,oxoglutaratebinding (Arg367) site. There is one alternative start site at Met34 that generates a 40kD isoform. In addition, there is another potential splice form that shows a 16aa substitution for aa1281 fulllength human EGLN2 shares 91% aa sequence identity with mouse EGLN2.
Catalog #P3373-02
ApplicationsSuitable for use in Western Blot and Immunoprecipitation.
Recommended DilutionWestern Blot: 1ug/ml
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS with Trehalose. Reconstitute with sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to S. frugiperda insect ovarian cell line human EGLN2/PHD1 expressed in Sf 21 cells (Q96KS0).
SpecificityRecognizes human EGLN2/PHD1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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