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You are here:Home » Antibodies » Abs to Hypoxia » Anti -PHD1, IN (Egl Nine Homolog 2, Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF-prolyl Hydroxylase 1, HIF-PH1, HPH-3, Prolyl Hydroxylase Domain-containing Protein 1, EGLN2, Estrogen-induced Tag 6, EIT6)

Anti -PHD1, IN (Egl Nine Homolog 2, Hypoxia-inducible Factor Prolyl Hydroxylase 1,
HIF-prolyl Hydroxylase 1, HIF-PH1, HPH-3, Prolyl Hydroxylase Domain-containing Protein 1,
EGLN2, Estrogen-induced Tag 6, EIT6)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
PHD1 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.
Catalog #P3373-05E
ApplicationsSuitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:50-1:100. Formalin-fixed and paraffin-embedded human testis tissue reacted with P3373-05E, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
ELISA: 1:1000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography
ImmunogenSynthetic peptide selected from the Center region of human PHD1 conjugated to KLH.
SpecificityRecognizes human PHD1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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