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You are here:Home » Antibodies » Antibodies-Hypoxia » Anti -PHD1-3, pan (HIF-prolyl Hydroxylase 1-3)

Anti -PHD1-3, pan (HIF-prolyl Hydroxylase 1-3)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline.
Catalog #P3373A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:100,000; Using 50-100ng of P3373B control peptide/well.
Western Blot: 1-5ug/ml. Using ECL technique.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 18 amino acids near the N terminus of human PHD2/EGLN1.
SpecificityRecognizes human PHD1-3. Species Sequence Homology: mouse, rat, PHD2/EGLN1-100%; human, mouse, rat PHD1/EGLN2, 94%; human, mouse, rat PHD3/EGLN3, 88%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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