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You are here:Home » Antibodies » Abs to Hypoxia » Anti -PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HI

Anti -PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open
Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine
(C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HI

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex.
Catalog #P3374-01C
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormAs reported
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide made to an N-terminal sequence of the human EGL9 homolog 1 (between residues 1-50). [LocusLink ID 54583].
SpecificityThis antibody is specific for human HIF prolyl hydroxylase 2. Species Crossreactivity: Human. This antibody does not appear to work in mouse. Results have been mixed in rat with success in western analysis and immunofluorescence on rat endothelial cells and negative results with PC12 cells. Other species have not been tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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