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You are here:Home » Antibodies » Abs to Hypoxia » Anti -PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HI

Anti -PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open
Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine
(C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HI

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH FC
HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
Catalog #P3374-01D
ApplicationsSuitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry.. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10-1:100
Western Blot: 1:500-1:2500. Detects a band at 46-50kD
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
SpecificityRecognizes human PHD2. Recognizes an epitope mapped to a region between residues 1 and 50 of human PHD2 (NP_071334.1) (GeneID 54583). Does not react with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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