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You are here:Home » Antibodies » Abs to Hypoxia » Anti -PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase 2, SM-20)

Anti -PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl
Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12,
PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase 2, SM-20)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IF
PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. EGLN family, which is known to be cellular oxygen sensor, initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.
Catalog #P3374-03
ApplicationsSuitable for use in Immunofluorescence, ELISA, and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:500-1:1,000
Western Blot:1:500-1:1,000
Optimal dilutions to be determined by the researcher.
HybridomaMouse F0 myeloma cells with spleen cells from BALB/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No10K404
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenRecombinant human PHD2 (aa271-426) purified from E.coli (Accession #NP_071334).
SpecificityRecognizes human PHD2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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