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You are here:Home » Biochemicals » Biochem-Antibiotics » Phleomycin, Streptomyces verticillus

Phleomycin, Streptomyces verticillus

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Specifications

This unique antibiotic shows high toxicity in mammalian cells, plants, yeasts and bacteria. Ocin acts by binding to DNA and cleaving it causing cell death. Ocin is a copper-chelated glycopeptide antibiotic isolated from culture broth of a Streptomyces verticullus mutant and is part of the structurally related group of Bleomycin/Phleomycin type antibiotics.
Catalog #P4070-81
The product of Sh ble, (Streptoalloteichus hindustanus bleomycin-resistance) gene confers resistance to Zeocin and Zeocell. The 14kD protein stoichiometrically binds to Zeocin and inhibits DNA strand cleavage and subsequent cell death.
Produced by a Streptomyces verticullus mutant and is part of the structurally related group of Bleomycin/Phleomycin type antibiotics. Used as a selective agent for the incorporation of the Sh ble gene which encodes a 13,665 Dalton protein. The protein binds to the antibiotic and prevents binding to DNA.
Phleomycin is a glycopeptide antibiotic of the bleomycin family, isolated from a mutant strain of Streptomyces verticullus. It binds and intercalates DNA thus destroying the integrity of the double helix. Use of phleomycin is recommended for cells poorly sensitive to Zeocin. Phleomycin is active against most bacteria, filamentous fungi, yeast, plant and animal cells.
ApplicationsUsed as a selective agent for the incorporation of the Sh ble gene which encodes a 13.665kD protein. The protein binds to the antibiotic and prevents binding to DNA.
Bacilllus Subtilis 168:
1.5-4.5ug/ml
E.ColiMC1061 sensitive strain: 1.5-4.5ug/ml
MC1061 resistant strain: 1500-4500ug/ml
Top 10 sensitive strain: 1.5-4.5ug/ml
Top 10-pCDNA3Zeo (resistant strain): 1500-4500ug/ml
Pichia pastoris X331.5-4.5ug/ml
SaccharomycesOL1 sensitive strain: 0.45-1.5ug/ml
OL1 resistant strain: >300ug/ml
Filmaentous FungiTrichoderma reesie sensitive strain: <30ug/ml
Animal Cell Lines293PS: 50-100ug/ml
B16: 50ug/ml
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number11006-33-0
Molecular FormulaC55H83N19O21S2Cu
Molecular Weight1137.4
Purity~90%
FormSupplied as a lyophilized powder
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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