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Specifications

This unique antibiotic shows high toxicity in mammalian cells, plants, yeasts and bacteria. Ocin acts by binding to DNA and cleaving it causing cell death. Ocin is a copper-chelated glycopeptide antibiotic isolated from culture broth of a Streptomyces verticullus mutant and is part of the structurally related group of Bleomycin/Phleomycin type antibiotics.
Catalog #	P4070-81
	The product of Sh ble, (Streptoalloteichus hindustanus bleomycin-resistance) gene confers resistance to Zeocin and Zeocell. The 14kD protein stoichiometrically binds to Zeocin and inhibits DNA strand cleavage and subsequent cell death.
	Produced by a Streptomyces verticullus mutant and is part of the structurally related group of Bleomycin/Phleomycin type antibiotics. Used as a selective agent for the incorporation of the Sh ble gene which encodes a 13,665 Dalton protein. The protein binds to the antibiotic and prevents binding to DNA.
	Phleomycin is a glycopeptide antibiotic of the bleomycin family, isolated from a mutant strain of Streptomyces verticullus. It binds and intercalates DNA thus destroying the integrity of the double helix. Use of phleomycin is recommended for cells poorly sensitive to Zeocin. Phleomycin is active against most bacteria, filamentous fungi, yeast, plant and animal cells.
Applications	Used as a selective agent for the incorporation of the Sh ble gene which encodes a 13.665kD protein. The protein binds to the antibiotic and prevents binding to DNA.
	Suggested Concentration for use:
	Organism:
	E. coli: 25-50ug/ml in Low Salt LB medium
	Yeast: 50-300ug/ml in YPD or minimal medium
	Mammalian Cells: 25-1000ug/ml (varies with cell line)
Solubility	Soluble in water.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for up to 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Long term stability in solution has not been evaluated for this product. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	11006-33-0
Molecular Formula	C55H83N19O21S2Cu
Molecular Weight	1137.4
Purity	~90%
Form	Supplied as a lyophilized powder.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.