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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Phosphatase, Alkaline, Chicken Intestine

Phosphatase, Alkaline, Chicken Intestine

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Specifications

Alkaline phosphatase is a broad term associated with non-specific phosphomonesterases with activity optima at alkaline pH.
Catalog #P4071-02
Activity 0.9 units per mg dry weight ( 25ºC, pH 8.8)
Unit Definition1 unit hydrolyzes 1umole of o-carboxyphenyl phosphate per minute at 25ºC, pH 8.8.
CompositionThe enzyme is a zinc metallo-enzyme. Schüssler (1968) reports four isozymes. Chang and Moog (1972) found three isozymes in the enzyme from chicken duodenum (This preparation is from the whole intestine.)
Optimum pH 8-9
A280@mg/ml As Reported
Activators Schüssler (1968) indicates activation by Mg2+. See Sivanaesan et al. (1991).
Inhibitors Acidification to pH 4.5 reversibly inactivates the enzyme.
Quality ControlSDS-PAGE
Storage and Stability:
Lyophilized powder may be stored at 4°C. Stable for 6 months at 4°C.
SourceChicken Intestine
PurityThis enzyme was partially purified and studied by Kunitz (1960).
FormSupplied as a lyophilized powder
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.1.3.1


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