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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Phosphatase, Alkaline, Calf Intestine, Solution (~3000u/mg)

Phosphatase, Alkaline, Calf Intestine, Solution (~3000u/mg)


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Alkaline Phosphatase is used to dephosphorylate dsDNA and RNA in molecular cloning and DNA sequencing reactions.
Catalog #P4071-03
CAS Number9001-78-9
SourceCalf Intestine
Prepared from US origin starting material and collected in USDA approved facilities
Protein Concentration
Assay Method
Based on the method of Garen and Levinthal whereby the reaction velocity is derived by measuring the absorbance increase at 410nm after hydrolysis of p-nitrophenyl phosphate.
Supplied as a liquid in deionized water.
~3000 units/mg protein (glycine units). 1 glycine unit = 2-3 DEA units
Unit Definition
One unit of enzyme hydrolyzes 1umole of p-nitrophenyl phosphate per minute at 37°C, pH 9.8 in the presence of Mg and Zn.
Quality Control
No contaminating RNase, DNase or Proteases detected
Storage and Stability
Stable for 1 year at 2-8ºC
Alternate namesEC=; Phosphoric monoester phosphorylase

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