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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Phosphatase, Alkaline, Calf Intestine, Solution (~3000u/mg)

Phosphatase, Alkaline, Calf Intestine, Solution (~3000u/mg)

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Specifications

Alkaline Phosphatase is used to dephosphorylate dsDNA and RNA in molecular cloning and DNA sequencing reactions.
Catalog #P4071-03
CAS Number9001-78-9
SourceCalf Intestine
Prepared from US origin starting material and collected in USDA approved facilities
Protein Concentration
1mg/ml
Assay Method
Based on the method of Garen and Levinthal whereby the reaction velocity is derived by measuring the absorbance increase at 410nm after hydrolysis of p-nitrophenyl phosphate.
Form
Supplied as a liquid in deionized water.
Activity
~3000 units/mg protein (glycine units). 1 glycine unit = 2-3 DEA units
Unit Definition
One unit of enzyme hydrolyzes 1umole of p-nitrophenyl phosphate per minute at 37°C, pH 9.8 in the presence of Mg and Zn.
Quality Control
SDS-PAGE
No contaminating RNase, DNase or Proteases detected
Storage and Stability
Stable for 1 year at 2-8ºC
Alternate namesEC=3.1.3.1; Phosphoric monoester phosphorylase


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