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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Phosphatase, Alkaline, Calf Intestine Conjugation Grade

Phosphatase, Alkaline, Calf Intestine Conjugation Grade

Pricing

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Specifications

Alkaline Phosphatase is used to dephosphorylate dsDNA and RNA in molecular cloning and DNA sequencing reactions. This is a cost effective conjugation grade used in antibody preparation supplied as a lyophilized powder.
Catalog #P4071-03B
CAS Number9001-78-9
SourceCalf Intestine
Prepared from US origin starting material and collected in USDA approved facilities
Form
Supplied as a lyophilized powder.
Purity
95%
Activity
1,500-2,500 units/mg protein
Unit Definition
The amount of enzyme which liberates 1umole of p-nitrophenol from p-nitrophenyl phosphate per minute at 37°C, pH 9.8 in diethanolamine buffer.
Quality Control
SDS-PAGE
No contaminating RNase, DNase or Proteases detected
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Country of Origin
Prepared from US origin starting material and collected in USDA approved facilities.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with distilled water or sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Alternate namesEC=3.1.3.1; Phosphoric monoester phosphorylase


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