Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Phosphatase, Alkaline (Alkaline Phosphatase, AP) (Biotin)

Phosphatase, Alkaline (Alkaline Phosphatase, AP) (Biotin)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

The alkaline phosphatase used for this product is isolated from calf intestine. The very high specific activity of this enzyme is retained during biotinylation procedure. Biotinylated alkaline phosphatase used in conjunction with Avidin D provides one of the most sensitive enzyme detection systems available. For some solid-phase assay systems, the use of biotinylated alkaline phosphatase provides a more sensitive system than the Avidin D conjugate. This enzyme also offers a potential for use as a second marker in immunohistochemical staining.
Catalog #P4071-38B
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Activity2200 units/mg protein. One unit = 1 umole of p-nitrophenol liberated from p-nitrophenylphosphate per minute t 37C (pH 9.5)
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Concentration~1mg/ml
FormSupplied as a liquid in 10mM HEPES, 015M sodium chloride, 1mM mMg++, 1mM Zn++, pH 7.5, 0.08% azide. Labeled with Biotin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links