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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -Alkaline Phosphatase

Anti -Alkaline Phosphatase

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). In vivo studies have identified CIAP as a potential therapeutic agent for the treatment of lipopolysaccharide (LPS)-mediated inflammation and sepsis, due to the ability of CIAP to neutralise and thereby detoxify (LPS).
Catalog #P4071-39
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution
ELISA: 1:1000 -1:10000
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1903-30U IgG, H&L, X-adsorbed (HRP) ShxRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Immunoaffinity chromatography.
ImmunogenHighly pure calf intestinal alkaline phosphatase.
SpecificityRecognizes calf intestinal alkaline phosphatase- (CIAP), a membrane-bound hydrolase enzyme highly expressed by intestinal mucosa. Species crossreactivity: porcine. Detects a band of ~67kD in porcine microvillar membranes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesIntestinal Alkaline Phosphatase


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