Anti -Phosphodiesterase 4B (PDE4B, PDEIVB, cAMP-specific 3',5'-cyclic Phosphodiesterase
4B, DPDE4, PDE32)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IC IF |
|
| PDE4B is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. Four genes (PDE4A, B, C and D) make up the PDE4 family. cAMP-dependent phosphodiesterase type B (PDE4B) family consists of four known splice variants, PDE4B1, B2, B3 and a novel 66kD PDE4B4 protein. One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In CNS, all four PDE4B subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Some PDE4B variants are also substrate for PKA-dependent phosphorylation. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants; the PDE4B4 (66kD protein) is the major PDE4B variant expressed in lungs, testis and heart. In testis PDE4B enzymes are expressed in somatic cells (Sertoli and Leydig cells) while PDE4A is localized in germ cells. | | | Catalog # | P4072-03H | | Applications | Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:200 | | Western Blot: 1:1500 detects a band at 107kD (PDE4B1), 78kD (PDE4B2), 100kD (PDE4B3) and 66kD (PDE4B4). | | Immunoprecipitation: 1:350 | | Immunocytochemistry: 1:300 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, 0.02% thimerosal, 20% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to the N-terminal of human Phosphodiesterase 4B. | | Specificity | Recognizes human Phosphodiesterase 4B. Reacts with all PDE4B proteins including PDE4B1, PDE4B2, PDE4B3 and a 66kD PDE4B variant. Does not crossreact with PDE4A, PDE4C or PDE4D. | | Species Crossreactivity | Mouse, rat, chicken and primate | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Phosphodiesterase 4B, cAMP-specific, DKFZp686F2182, EC=3.1.4.17, MGC126529, PDE4B5 |
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