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You are here:Home » Antibodies » Abs to Enzymes, Phosphodiesterase » Anti -Phosphodiesterase 5A (Phosphodiesterase 5A cGMP-specific, PDE5A, PDE-5A, PDE5A1, cGMP Binding cGMP Specific 3' 5' Cyclic Nucleotide Phosphodiesterase, cGMP-binding cGMP-specific 3 5-cyclic Nucleotide Phosphodiesterase, cGMP Binding cGMP Specific Phospho

Anti -Phosphodiesterase 5A (Phosphodiesterase 5A cGMP-specific, PDE5A, PDE-5A, PDE5A1,
cGMP Binding cGMP Specific 3' 5' Cyclic Nucleotide Phosphodiesterase, cGMP-binding
cGMP-specific 3 5-cyclic Nucleotide Phosphodiesterase, cGMP Binding cGMP Specific
Phospho

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The cyclic monophosphate nucleotides are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A. The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP-dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB-PDEs). The cGB-PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site.
Catalog #P4072-05B
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Immunoprecipitation: 1:250
Optimal dilutions to be determined by the researcher.
Positive ControlRat lung homogenate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic cyclic peptide corresponding to rat Phosphodiesterase 5A Sequence homology
SpecificityRecognizes rat Phosphodiesterase 5A. Detects 99kD and 89kD PDE5A proteins in various tissues. Species Crossreactivity: human and mouse .
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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