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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IF

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.
Catalog #	P4073-52
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Can also be used to increase the calcium uptake in ventricular SR vesicles analogous to the phosphorylation of PLB following adrenergic stimulation.
	Other applications not tested.
Recommended Dilution	Immunofluorescence: 20ug/ml; Immunofluorescence staining of PLB in rat papillary muscle tissue results in diffuse staining of the network sarcoplasmic reticulum (SR).
	Western Blot: 2ug/ml; Detects a 25kD protein under non-reducing conditions representing the pentameric form of PLB and a 5kD protein under reducing conditions representing the monomeric form of PLB in canine cardiac extracts.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	4i237
Host	Mouse
Source	Canine
Concentration	~1mg/ml
Form	Supplied as a liquid in MOPS/PBS with glycine, pH7.2, 0.05% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa 2-25, D2KVQYLTRSAIRRASTIEMPQQAR25 of canine PLB (phospholamban).
Specificity	Recognizes an epitope between amino acid residues 9-17 ofcanine PLB. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.