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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH IC

Catalog #	P4073-52A
Applications	Suitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot and most Functional applications. Other applications not tested.
	Recommended Dilutions:
	Immunocytochemistry: 20ug/ml
	Immunohistochemistry: Paraffin sections
	Western Blot: 2ug/ml Detects bands of ~25kD (non-reducing conditions; representing the pentameric form of PLB) and ~5kD (reducing conditions; representing the monomeric form of PLB).
	Functional Applications: Increases the calcium uptake in ventricular SR vesicles.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	6D657
Host	Mouse
Source	Canine
Concentration	Not Determined
Form	Supplied as a liquid in PBS, 0.05% sodium azide.
Purity	Ascites
Immunogen	Synthetic peptide corresponding to aa 2-25 of canine PLB (phospholamban), D2KVQYLTRSAIRRASTIEMPQQAR25.
Specificity	Recognizes canine Phospholamban. Recognizes an epitope between aa9-17 of canine PLB. Species Crossreactivity: human, mouse, rat, rabbit, chicken, sheep, porcine, guinea pig
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.