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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase A2, Secretory, Group IB (PLA2G1B, phospholipase A2, MGC119834, MGC119835, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 precursor, PLA2, PLA2A, PPLA2)

Anti -Phospholipase A2, Secretory, Group IB (PLA2G1B, phospholipase A2, MGC119834,
MGC119835, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 precursor, PLA2, PLA2A,
PPLA2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
PLA2G1B, hydrolyses the sn-2 ester bond of phospholipids, including those in cell membranes, to generate non-esterified free fatty acids and lysophospholipids. Most secretory PLA2s are stored in cytoplasmic granules and are released into plasma and biological fluids on appropriate cell activation, especially during systemic inflammatory, autoimmune, or allergic disease. PLA2G1B is abundant in digestive organs but is also found elsewhere. Human PLA2G1B shares 78-76aa sequence identity with mouse and rat PLA2G1B, respectively.
Catalog #P4074-06D5
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 2ug/ml using human pancreas tissue under non-reducing conditions
Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human PLA2G1B.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No11n151
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-148 from human PLA2G1B expressed in NSO cells (NM_000928).
SpecificityRecognizes human PLA2G1B. No crossreactivity with recombinant human PLA2G2A, PLA2G7, recombinant mouse PLA2G2A or PLA2R1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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