Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -PLA2G2A (phospholipase A2, group IIA (platelets, synovial fluid)GIIC sPLA2, Group IIA phospholipase A2, MOM1, Non-pancreatic secretory phospholipase A2, NPS-PLA2, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2, membrane associated precursor, PLA2,

Anti -PLA2G2A (phospholipase A2, group IIA (platelets, synovial fluid)GIIC sPLA2, Group
IIA phospholipase A2, MOM1, Non-pancreatic secretory phospholipase A2, NPS-PLA2,
Phosphatidylcholine 2-acylhydrolase, Phospholipase A2, membrane associated precursor,
PLA2,

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IP
Phospholipases A2 (PLA2s) hydrolyze the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids. They play an important role in a variety of cellular processes, including digestion and metabolism of phospholipids, as well as production of precursors for inflammatory reactions. Phospholipases A2 belongs to PLA2 group II, which consists of extracellular enzymes with low molecular masses that require calcium ions for catalysis.
Catalog #P4074-06G4
ApplicationsSuitable for use in Direct ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human PLA2G2A is ~1.5ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human PLA2G2A is ~2ng/lane under non-reducing conditions and ~50ng/lane under reducing conditions.
Immunoprecipitation: Used to immunoprecipitate recombinant human PLA2G2A from conditioned media of transfected Sf21 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to aa21-144 of human PLA2G2A expressed in Sf21 cells.
SpecificityRecognizes human PLA2G2A. Species Crossreactivity: recombinant mouse PLA2G2A, 60%; recombinant human PLA2G1B and recombinant human PLA2G7, <5%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links