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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -PLA2G16 (Group XVI Phospholipase A2, Adipose-specific Phospholipase A2, AdPLA, H-rev 107 Protein Homolog, HRAS-like Suppressor 3, HREV107, HREV107-3, Renal Carcinoma Antigen NY-REN-65, HRASLS3)

Anti -PLA2G16 (Group XVI Phospholipase A2, Adipose-specific Phospholipase A2, AdPLA, H-rev
107 Protein Homolog, HRAS-like Suppressor 3, HREV107, HREV107-3, Renal Carcinoma Antigen
NY-REN-65, HRASLS3)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
PLA2G16 (Group 16 phospholipase A2; also HREV107 and AdPLA) is a Ca++-dependent 17-18kD member of the H-rev107 family of molecules. It is expressed intracellularly by white adipocytes, induced by insulin, and generates arachadonic acid from phospholipids. The arachadonic acid is converted to PGE2, which binds the EP3 receptor and decreases cAMP levels. This results in decreased hormone-sensitive lipase activity and increased adiposity. Human PLA216 is 16211aa in length. It is associated with both membranes and cytosol, and may possess a transmembrane segment between aa134-154. The enzymatic activity resides in His23 and Cys113. There is one potential isoform that shows a 20aa substitution for the first four amino acids. Over aa12-132, human PLA2G16 shares 88% aa identity with mouse PLA2G16.
Catalog #P4074-16
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA:0.5-1ug/ml
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in 100ul sterile water.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, E.coli-derived recombinant human PLA2G16. (Asp12-Asp132; Accession #P53816).
SpecificityRecognizes human PLA2G16.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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