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You are here:Home » Antibodies » Antibodies-Enzymes, Phospholipase » Anti -Phospholipase C, beta 3, phosphorylated (Ser537) (PLCb3)

Anti -Phospholipase C, beta 3, phosphorylated (Ser537) (PLCb3)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5- triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLC5, PLCbeta, PLCgamma, PLCdelta and PLCepsilon have been identified so far. The PLCbeta subfamily includes four members, PLCbeta14. All four members of the subfamily are activated by alphaor beta-gamma-subunits of the heterotrimeric G-proteins (2,3). Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of serine 1105 by PKA or PKC inhibits PLCbeta3 activity (4,5). Serine 537 of PLCbeta3 is phosphorylated by CaMKII and this phosphorylation may contribute to the basal activity of PLCbeta3. PLCgamma is activated by both receptor and nonreceptor tyrosine kinases (6). PLCgamma forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCgamma1 on tyrosine residues 771, 783 and 1245 (7). Phosphorylation by Syk at tyrosine 783 activates the enzymatic activity of PLCgamma1 (8).
Catalog #P4074-40J
Recommended Dilution Western Blotting: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding residues surrounding Ser537 of human PLCbeta3.
SpecificityDetects endogenous human PLCbeta3 only when phosphorylated at Ser537. Does not crossreact with phosphorylated PLCbeta1, PLCbeta2, PLCbeta4 or other PLCs.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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