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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -PLCD3 (1-phosphatidylinositol-4,5-bisphosphate Phosphodiesterase delta-3, Phosphoinositide Phospholipase C-delta-3, Phospholipase C-delta-3, PLC-delta-3, KIAA1964)

Anti -PLCD3 (1-phosphatidylinositol-4,5-bisphosphate Phosphodiesterase delta-3,
Phosphoinositide Phospholipase C-delta-3, Phospholipase C-delta-3, PLC-delta-3, KIAA1964)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
PLC-d3 (phospholipase C-delta 3) is an 105kD member of the d-type PLC isozyme family. It is expressed in corneal epithelium, platelets, cardiac and skeletal muscle. PLC-d3 preferentially hydrolyzes membrane PIP release, and DAG, activating PKC. Human PLC-d3 is 789aa in length. It contains a plextrin homology domain that tethers the enzyme to the membrane (aa63-172), three EF-hand motifs that spatially orient the molecule (aa182-285), a catalytic region (aa337-644), and a C2 domain that secures the catalytic region to its substrate (aa647-752). There are multiple potential isoforms. There is an alternate start site at Met54, a 40aa substitution for the C-terminal 248 aa, and a Leu substitution for aa102-109. Over aa148-344, human PLC-d3 is 93% aa identical to mouse PLC-d3.
Catalog #P4074-41C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot:1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium chloride.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, E. coli-derived recombinant human PLC-d3 (aa148-344; Accession #Q8N3E9).
SpecificityRecognizes human PLCD3. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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