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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase C, gamma 1 (PLCg1)

Anti -Phospholipase C, gamma 1 (PLCg1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IC
Phospholipase C gamma 1 is a tryosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-triphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLC gamma activity is unknown.
Catalog #P4074-42
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations.
Immunoprecipitation: 4ug Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation.
Immunocytochemistry: 5-10ug/ml detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells.
Optimal dilutions to be determined by the researcher.
Positive Antigen Control (Included) A0001-50 (Non-stimulated A431 cell lysate). Add 2.5ul of 20mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Use 20ug per lane for minigels.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile ddH20, 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No0.T.170
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a lyophilized powder from 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide. Reconstitute with 40ul dH2O, 40-50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenFive individual clones raised against purified soluble phospholipase C (PLC) g-1 from bovine brain.
SpecificityRecognizes bovine PLC g-1 at 135kD. Reacts with some non-PLC g-1 SH-3 containing proteins, including nck (47kD). Species crossreactivity: rabbit, rat, mouse and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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