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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase C, gamma 1 (PLCg1)

Anti -Phospholipase C, gamma 1 (PLCg1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Phospholipase C gamma 1 is a tryosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-triphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLC gamma activity is unknown.
Catalog #P4074-42C
ApplicationsSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.1-0.5ug/ml. Detects PLCg1 in acid precipitated protein from bovine brain, RIPA lysates from A431 cells, and lysates from 3Y3/A31 fibroblasts and L6 myoblasts.
Immunoprecipitation: 4ug (bovine brain cytosol).
Optimal dilutions to be determined by the researcher.
Positive Antigen Control (Included) A0001-50 (Non-stimulated A431 cell lysate). Add 2.5ul of 20mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Use 20ug per lane for minigels.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding ddH2O and 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.117
HostMouse
SourceBovine
Concentration1mg/ml
FormSupplied as lyophilized powder from 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide. Reconstitute with 100ul dH2O, 40-50% glycerol.
PurityPurified by Protein G chromatography.
ImmunogenPurified soluble phospholipase C (PLC) g-1 from bovine brain.
SpecificityRecognizes bovine PLCg-1 at ~135kD. May also react with some non-PLC g-1 SH-3 containing proteins(4). Species crossreactivity: rabbit, rat, mouse and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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