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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase C, gamma 1, phosphorylated (Tyr783) (PLCg1)

Anti -Phospholipase C, gamma 1, phosphorylated (Tyr783) (PLCg1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.
Catalog #P4074-42F
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1,000
Optimal dilutions to be determined by the researcher.
Positive ControlA431 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenLinear peptide corresponding to phospho-PLCg-1 (Tyr783) (KLH).
SpecificityRecognizes human Phospholipase C, gamma 1, Phosphorylated (Tyr783). Species sequence homology: Mouse, Rat, Equine, Canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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